Original Article

홍국발효 柴胡가 Lipopolysaccharide로 유발된 급성 폐 손상에 미치는 영향

Effects of Red Koji-Fermented Bupleuri Radix Extracts on Lipopolysaccharide-Induced Rat Acute Lung Injury

2021 Vol. 13 No. 1
서영호1 · 정태영2 · 김종대3 · 최해윤3

경상북도 포항시 북구 천마로 75 서영호한의원1

대구한의대학교 한의과대학 진단학교실2

대구한의대학교 한의과대학 내과학교실3
Young-ho Seo1 · Hae-yun Choi2 · Jong-dea Kim2 · Tae-young Jung3

Seo-Young-Ho Korean Medicine Clinic1

Dept. of Diagnostics, College of Korean Medicine, Daegu Haany University2

Dept. of Internal Medicine, College of Korean Medicine, Daegu Haany University3


ObjectiveThis study aimed to assess the preventive effect of Bupleuri Radix aqueous extracts (BR) and red koji-fermented BR (fBR) in lipopolysaccharide (LPS)-induced acute lung injury in a rat model.

MethodsRats were administered 30, 60, or 120 mg/kg/day of fBR for 28 days before LPS treatments. All rats were sacrificed 5 h after LPS treatment (500 μg/head, intratracheal instillation). Body weights, lung weights, pulmonary transcapillary albumin transit, arterial gas parameters (pH, partial pressure [Pa] of O2, PaCO2), bronchoalveolar lavage fluid (BALF) protein, lactate dehydrogenase (LDH), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), total cell numbers, neutrophil/alveolar macrophage ratios, lung malondialdehyde (MDA), and myeloperoxidase (MPO) were measured. In addition, histopathological changes including the luminal surface of alveoli (LSA), thickness of alveolar septum, and number of polymorphonuclear neutrophils (PMNs) were checked.

ResultsLPS injection led to increases in lung weights, pulmonary transcapillary albumin transit, BALF protein, LDH, TNF-α and IL-1β contents, total cells, neutrophil and alveolar macrophage ratios, lung MDA, MPO, alveolar septum thickness, and PMNs, and decreases in PaCO2 and pH of arterial blood and LSA. However, these LPS-induced acute lung injuries were inhibited by pretreatment of 30, 60, and 120 mg/kg of fBR. The most favorable effects were seen with 30 mg/kg fBR as compared with 60 mg/kg of α-lipoic acid and BR.

ConclusionsfBR showed preventive effects on LPS-induced acute lung injury, which resembles acute respiratory distress syndrome. The mechanisms of action were likely via antioxidant and anti-inflammatory means.

Key wordsRed koji-fermented Bupleuri Radix, lipopolysaccharide, LPS, acute lung injury